FUS, EWS, and TAF15 Proteins in Human Tissues
Author Information
Author(s): Andersson Mattias K, Ståhlberg Anders, Arvidsson Yvonne, Olofsson Anita, Semb Henrik, Stenman Göran, Nilsson Ola, Åman Pierre
Primary Institution: Lundberg Laboratory for Cancer Research, Department of Pathology, Sahlgrenska Academy at Göteborg University, Göteborg, Sweden
Hypothesis
The study investigates the expression and cellular localization of FET proteins in multiple human tissues and cell types.
Conclusion
The FET proteins exhibit cell-specific expression patterns and functions, suggesting their roles in stress response and cell adhesion.
Supporting Evidence
- FUS, EWS, and TAF15 were expressed in both distinct and overlapping patterns in human tissues.
- The proteins showed almost ubiquitous nuclear expression.
- FUS and TAF15 were present in the cytoplasm of most cell types.
- FET expression was downregulated in differentiating human embryonic stem cells.
- The FET proteins were targeted to stress granules induced by heat shock and oxidative stress.
- FUS and TAF15 were detected in spreading initiation centers of adhering cells.
Takeaway
This study looks at how certain proteins behave in different types of human cells, showing that they can act differently depending on the cell type.
Methodology
Immunostaining and ectopic expression of proteins were used to examine the expression patterns of FET family members in human tissues.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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