Mapping the Genetic Architecture of the Adaptive Integrated Stress Response in Yeast
Author Information
Author(s): Rachel Baum, Jinyoung Kim, Ryan Y. Muller, Ingolia Nicholas T.
Primary Institution: University of California, Berkeley
Hypothesis
How do genetic perturbations change the dynamics of the integrated stress response (ISR) in budding yeast?
Conclusion
The study illustrates that ribosome levels impact ISR dynamics, with ribosome biogenesis mutants showing altered activation patterns under stress.
Supporting Evidence
- Disrupting ribosome biogenesis genes shifted ISR dynamics.
- Lower doses of sulfometuron methyl caused transient ISR activation followed by growth recovery.
- High doses of sulfometuron methyl led to persistent ISR activation and growth arrest.
Takeaway
This study looks at how yeast cells respond to stress and how changes in their genes can affect that response, especially when they are low on amino acids.
Methodology
The researchers used CRISPR interference with barcoded expression reporter sequencing (CiBER-seq) to profile ISR dynamics across thousands of genetic perturbations.
Limitations
The study primarily focuses on yeast, which may limit the generalizability of the findings to other organisms.
Digital Object Identifier (DOI)
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