Identifying Genes in the Maize Pathogen Cercospora zeae-maydis
Author Information
Author(s): Bluhm Burton H, Dhillon Braham, Lindquist Erika A, Kema Gert HJ, Goodwin Stephen B, Dunkle Larry D
Primary Institution: Department of Plant Pathology, University of Arkansas
Hypothesis
What are the genes expressed during different growth stages of Cercospora zeae-maydis?
Conclusion
The study identified novel genes involved in growth, development, and pathogenesis in Cercospora zeae-maydis, which can be targeted for further research.
Supporting Evidence
- The study generated 27,551 expressed sequence tags (ESTs) from five cDNA libraries.
- Identified 4,619 putative unique genes, with 36% similar to known proteins.
- Real-time PCR confirmed the differential expression of selected genes during growth stages.
Takeaway
Scientists studied a fungus that harms corn plants to find new genes that help it grow and cause disease.
Methodology
The study involved generating cDNA libraries from different growth conditions of the fungus and sequencing expressed sequence tags (ESTs).
Limitations
The study's findings may be limited by the quality and representativeness of the cDNA libraries constructed.
Digital Object Identifier (DOI)
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