Bisphosphonates regulate cell growth and gene expression in the UMR 106-01 clonal rat osteosarcoma cell line
2001
Effects of Bisphosphonates on Osteosarcoma Cells
publication
Evidence: moderate
Author Information
Author(s): Mackie P S, Fisher J L, Zhou H, Choong P F M
Primary Institution: St. Vincent's Hospital, Fitzroy, Melbourne, Victoria, Australia
Hypothesis
Do bisphosphonates affect cell growth and gene expression in osteosarcoma cells?
Conclusion
Bisphosphonates inhibit osteosarcoma cell growth and induce apoptosis while altering the expression of osteoclast-regulating factors.
Supporting Evidence
- Both bisphosphonates inhibited cell proliferation in a time- and dose-dependent manner.
- Increased DNA fragmentation was observed in bisphosphonate-treated cultures.
- Morphological changes of apoptosis were more pronounced in bisphosphonate-treated cells.
- mRNA expression of osteopontin and RANKL was down-regulated by both bisphosphonates.
Takeaway
This study shows that certain drugs can help slow down cancer cell growth and make them die off more easily.
Methodology
In vitro study using UMR 106-01 rat osteosarcoma cell line with treatments of bisphosphonates and assessments of cell proliferation, apoptosis, and mRNA expression.
Digital Object Identifier (DOI)
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