Activation of Protein Kinase C Induces Differentiation in Human T-Lymphoblastic Cells
Author Information
Author(s): Y. Yamauchi, K. Nagasawa, T. Mayumi, T. Horiuchi, Y. Niho
Primary Institution: First Department of Internal Medicine, Faculty of Medicine, Kyushu University
Hypothesis
Does activation of protein kinase C (PKC) induce differentiation in the human T-lymphoblastic cell line MOLT-3?
Conclusion
The study suggests that the quantity of cytosolic PKC activity and the extent of translocation are related to responses to TPA-induced cell differentiation in the T-cell line MOLT-3.
Supporting Evidence
- TPA treatment led to a rapid decrease in PKC activities in the cytosol and an increase in the particulate fraction.
- The amount of PKC translocated in TPA-resistant subclones was about 20% of that in parental MOLT-3 cells.
- TPA-resistant subclones maintained high levels of terminal deoxynucleotidyl transferase activity even in the presence of TPA.
Takeaway
When a special protein called PKC is activated, it helps certain cells grow up and change into different types, but some cells resist this change.
Methodology
The study involved assaying PKC activities in MOLT-3 and its TPA-resistant subclones, examining the effects of TPA on PKC translocation and differentiation markers.
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