Role of CD151, A tetraspanin, in porcine reproductive and respiratory syndrome virus infection
2007
Role of CD151 in PRRSV Infection
publication
Evidence: moderate
Author Information
Author(s): Shanmukhappa Kumar, Kim Jeong-Ki, Kapil Sanjay
Primary Institution: Cincinnati Children's Hospital Medical Center, University of Cincinnati
Hypothesis
We sought to identify the host cellular proteins interacting with PRRSV 3' UTR RNA.
Conclusion
CD151 plays a critical role in PRRSV infection in vitro.
Supporting Evidence
- CD151 interacts with PRRSV 3' UTR RNA.
- Transfection of CD151 into non-susceptible cells made them susceptible to PRRSV.
- Blocking CD151 with antibodies completely inhibited PRRSV infection.
Takeaway
CD151 is a protein that helps the PRRS virus infect cells, and blocking it can stop the virus from spreading.
Methodology
We constructed a cDNA library from MARC-145 cells and screened it to identify proteins that bind to PRRSV 3' UTR RNA.
Digital Object Identifier (DOI)
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