Resistance to bile acids in colon cancer cells
Author Information
Author(s): Ashley A. Powell, Sandeep Akare, Wenqing Qi, Pascal Herzer, Samira Jean-Louis, Rebecca A. Feldman, Jesse D. Martinez
Primary Institution: University of Arizona, Arizona Cancer Center
Hypothesis
Is there an overlap in signaling pathways activated by tumorogenic bile acid deoxycholic acid (DCA) and chemopreventive bile acid ursodeoxycholic acid (UDCA)?
Conclusion
The study concludes that UDCA and DCA have overlapping signaling activities, and that disruption of these pathways can lead to a more advanced neoplastic phenotype.
Supporting Evidence
- 88% of HOMUR lines showed reduced apoptosis to DCA compared to parental HCT116 cells.
- 93% of HOMUR lines exhibited reduced apoptosis induced by hyoDCA.
- HOMUR 7 cells showed no evidence of apoptosis when treated with hyoDCA.
Takeaway
The study found that some cancer cells that resist one bile acid also resist another, which might make them more likely to grow into tumors.
Methodology
The study involved mutagenizing HCT116 cells to isolate lines resistant to UDCA-induced growth arrest and testing their response to DCA-induced apoptosis.
Statistical Information
P-Value
<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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