Ofd2: A Nuclear Dioxygenase That Interacts with Histones
Author Information
Author(s): Korvald Hanne, Mølstad Moe Anne Margrethe, Cederkvist F. Henning, Thiede Bernd, Laerdahl Jon K., Bjørås Magnar, Alseth Ingrun
Primary Institution: Oslo University Hospital HF Rikshospitalet
Hypothesis
What is the role of the Ofd2 dioxygenase in Schizosaccharomyces pombe?
Conclusion
Ofd2 is an active 2-oxoglutarate and iron-dependent dioxygenase that interacts with histones, particularly stimulating succinate formation.
Supporting Evidence
- Ofd2 is categorized as an AlkB homolog but does not exhibit DNA repair activity.
- Histones stimulate the decarboxylation activity of Ofd2, particularly H2A.
- Ofd2 interacts with all four core histones, but only weakly with H4.
Takeaway
Ofd2 is a protein in yeast that helps convert a molecule called 2-oxoglutarate into succinate, especially when it interacts with histones, which are proteins that help package DNA.
Methodology
The study involved expressing and purifying Ofd2 in E. coli, followed by assays to measure succinate production in the presence of histones.
Limitations
The study did not identify specific histone modifications required for Ofd2 activity.
Digital Object Identifier (DOI)
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