How Neuropilin-1 Helps Endothelial Cells Stick to Fibronectin
Author Information
Author(s): Valdembri Donatella, Caswell Patrick T, Anderson Kurt I, Schwarz Juliane P, König Ireen, Astanina Elena, Caccavari Francesca, Norman Jim C, Humphries Martin J, Bussolino Federico, Serini Guido
Primary Institution: University of Torino School of Medicine
Hypothesis
Neuropilin-1 regulates α5β1 integrin traffic and function in endothelial cells.
Conclusion
Neuropilin-1 enhances endothelial cell adhesion to fibronectin by promoting the internalization and recycling of active α5β1 integrin.
Supporting Evidence
- Neuropilin-1 is required for endothelial cell response to VEGF-A isoforms.
- Nrp1 promotes α5β1 integrin-mediated adhesion to fibronectin.
- Nrp1 interacts with GIPC1 to stimulate the internalization of active α5β1 integrin.
- Loss of Nrp1 leads to impaired endothelial cell adhesion and fibrillogenesis.
Takeaway
Neuropilin-1 helps cells stick to a protein called fibronectin, which is important for blood vessel development.
Methodology
The study used RNA interference to silence Neuropilin-1 in endothelial cells and assessed the effects on cell adhesion and integrin trafficking.
Digital Object Identifier (DOI)
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