Molecular and computational analysis of a novel pathogenic variant in emopamil-binding protein (EBP) involved in cholesterol biosynthetic pathway causing a rare male EBP disorder with neurologic defects (MEND syndrome)
2025

Study of a New Genetic Variant in EBP Linked to MEND Syndrome

Sample size: 2 publication Evidence: moderate

Author Information

Author(s): Bibi Hadiba, Ahmad Riaz, Rahman Fatima, Maqbool Shazia, Naeem Muhammad, Efthymiou Stephanie, Houlden Henry

Primary Institution: Quaid-i-Azam University, Islamabad, Pakistan

Hypothesis

The study aims to investigate disease-causing variants in a family affected by MEND syndrome.

Conclusion

The study identifies a novel pathogenic variant in the EBP gene associated with MEND syndrome, expanding the known mutation spectrum.

Supporting Evidence

  • The identified variant NM_006579.3:c.556T>C was found segregating in the affected family.
  • In-silico analysis supported the pathogenicity of the identified variant.
  • This is the first report of MEND syndrome from Pakistan.

Takeaway

This study found a new genetic change in a family with a rare disorder that affects how their bodies make cholesterol.

Methodology

Whole exome sequencing was performed on genomic DNA from two patients, followed by Sanger sequencing to confirm the findings.

Limitations

The study is limited by the small sample size and the rarity of the disorder.

Participant Demographics

Two male patients from a consanguineous family in Pakistan.

Digital Object Identifier (DOI)

10.1007/s11033-024-10183-7

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