How Transport Inhibitors Affect Nanoparticle Uptake in Different Cell Lines
Author Information
Author(s): Tiago dos Santos, Juan Varela, Iseult Lynch, Anna Salvati, Kenneth A. Dawson
Primary Institution: Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Ireland
Hypothesis
This study investigates the mechanisms by which carboxylated polystyrene nanoparticles are internalized by various human cell lines using pharmacological inhibitors.
Conclusion
The study found that nanoparticles can enter different cell types through various endocytic pathways, and their uptake is significantly influenced by the cytoskeleton and transport inhibitors.
Supporting Evidence
- Nanoparticle uptake was energy-dependent across all cell types studied.
- Different inhibitors affected nanoparticle uptake differently in each cell line.
- Actin depolymerization significantly reduced nanoparticle uptake in HeLa and 1321N1 cells.
Takeaway
This research shows that tiny particles can get into cells in different ways, and some medicines can stop them from doing so.
Methodology
The study used flow cytometry to measure nanoparticle uptake in HeLa, A549, and 1321N1 cells after treatment with various endocytosis inhibitors.
Limitations
The inhibitors used may not fully inhibit all endocytic pathways, and the study focused on only a few cell lines.
Participant Demographics
The study involved human cell lines: HeLa (cervical cancer), A549 (lung carcinoma), and 1321N1 (brain astrocytoma).
Digital Object Identifier (DOI)
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