Detailed deletion mapping of chromosome band 14q32 in human neuroblastoma defines a 1.1-Mb region of common allelic loss
2000
Mapping Chromosome 14 in Neuroblastoma
Sample size: 54
publication
Evidence: moderate
Author Information
Author(s): Hoshi M, Otagiri N, Shiwaku H O, Asakawa S, Shimizu N, Kaneko Y, Ohi R, Hayashi Y, Horii A
Primary Institution: Tohoku University School of Medicine
Hypothesis
What are the genetic contributions of abnormalities on chromosome 14 in neuroblastoma?
Conclusion
The study identified a 1.1-Mb region of common allelic loss on chromosome 14 in neuroblastoma samples.
Supporting Evidence
- 31% of the tumors showed loss of heterozygosity at one or more markers.
- The smallest common region of allelic loss was identified between D14S62 and D14S987.
- Fluorescence in situ hybridization confirmed the loss.
Takeaway
Researchers looked at 54 neuroblastoma samples to find a specific area on chromosome 14 that often had missing genetic information.
Methodology
Analyzed loss of heterozygosity in 54 primary neuroblastoma samples using 12 microsatellite markers.
Limitations
No statistical correlation was found between LOH and clinicopathologic features.
Participant Demographics
Infants with neuroblastoma.
Digital Object Identifier (DOI)
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