Evaluation of biolistic gene transfer methods in vivo using non-invasive bioluminescent imaging techniques

2011

Evaluating Gene Transfer Methods Using Imaging Techniques

Sample size: 24 publication 10 minutes Evidence: moderate

Author Information

Author(s): Xia Jixiang, Martinez Angela, Daniell Henry, Ebert Steven N

Primary Institution: Burnett School of Biomedical Sciences, University of Central Florida College of Medicine

Can biolistic gene transfer methods be effectively evaluated in vivo using non-invasive bioluminescent imaging techniques?

Biolistic gene transfer methods show different expression kinetics in skin and liver, with skin expression declining faster than liver expression.

Biolistic gene transfer to mouse skin produced peak reporter gene expression one day after transfer.
Expression in liver remained detectable for nearly two weeks post-transfer.
Different tissues showed different expression kinetics following gene transfer.

This study looked at how well a new way to deliver genes works in mice, finding that different body parts react differently to the treatment.

Mice were treated with plasmid DNA using a gene gun, and gene expression was measured using bioluminescent imaging.

Potential bias in the choice of gene delivery methods and the specific tissues evaluated.

The study was limited to only two types of tissues (skin and liver) and focused on short-term expression.

Adult white FVB mice, weighing 18-25 g.

p<0.05

p<0.05

Access the complete publication on the publisher's website