Reference Data Collection for Northern Blot Results
Author Information
Author(s): Danielle Kemmer, Margareta Faxen, Emily Hodges, Jonathan Lim, Elena Herzog, Elsebrit Ljungstrom, Anders Lundmark, Mary K. Olsen, Raf Podowski, Erik L. L. Sonnhammer, Peter Nilsson, Mark Reimers, Boris Lenhard, Steven L. Roberds, Claes Wahlestedt, Christer Hoog, Pankaj Agarwal, Wyeth W. Wasserman
Primary Institution: Center for Genomics and Bioinformatics, Karolinska Institutet, Stockholm, Sweden
Hypothesis
Can a reference data collection of Northern blot results facilitate the comparison of modern expression profiling techniques?
Conclusion
The study found that no modern profiling method produced results strongly correlated with Northern blot data, highlighting the need for multi-technique validation.
Supporting Evidence
- The study created a database of 619 Northern blots addressing 535 distinct genes.
- Correlation scores between microarray platforms and Northern blots were generally low.
- Microarrays performed better for genes with low expression levels compared to Northern blots.
Takeaway
This study created a database of Northern blot results to help scientists compare different ways of measuring gene expression, but found that none matched the traditional method very well.
Methodology
The study involved creating a database of Northern blot results and comparing them with data from various modern expression profiling techniques.
Potential Biases
The study may be biased towards genes with higher expression levels due to the nature of the Northern blot database.
Limitations
The correlation results between platforms were generally poor, indicating that the methods may not accurately reflect gene expression levels.
Digital Object Identifier (DOI)
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