Comparison of Affymetrix Exon and U133Plus2 GeneChip Arrays
Author Information
Author(s): Abdueva Diana, Wing Michele R., Schaub Betty, Triche Timothy J.
Primary Institution: Children's Hospital Los Angeles, Keck School of Medicine, University of Southern California
Hypothesis
How do the performance characteristics of Affymetrix exon arrays compare to conventional 3′-arrays?
Conclusion
The study found high comparability of expression changes and probe performance characteristics between Affymetrix conventional and exon arrays.
Supporting Evidence
- The study established high comparability of expression changes between the two platforms.
- HuEx arrays showed a smaller dynamic range and higher detection threshold compared to U133Plus2.
- The results provide a reliable benchmark for comparing exon expression measures.
Takeaway
This study looked at two types of gene chips to see how well they work for measuring gene activity, and found that they are quite similar.
Methodology
The study used spike-in hybridizations with 25 transcripts to compare the performance of HuEx and U133Plus2 arrays.
Limitations
The study does not address the performance of all possible summarization methods for exon arrays.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website