Downregulation and forced expression of EWS-Fli1 fusion gene results in changes in the expression of G 1 regulatory genes
2001
Effects of EWS-Fli1 Gene on Cell Cycle Regulation
publication
Author Information
Author(s): Matsumoto Y, Tanaka K, Nakatani F, Matsunobu T, Matsuda S, Iwamoto Y
Primary Institution: Department of Orthopaedic Surgery, Graduate School of Medical Sciences, Kyushu University
Hypothesis
EWS-Fli1 may affect the expression of G 1 regulatory genes.
Conclusion
The study suggests that EWS-Fli1 influences the expression of G 1 regulatory genes, which may contribute to tumorigenesis in Ewing tumors.
Supporting Evidence
- Downregulation of EWS-Fli1 fusion proteins was observed 48 hours after treatment.
- Expressions of G 1 cyclins were markedly decreased with reduced EWS-Fli1 fusion protein.
- The expression of p21 and p27 increased dramatically after treatment.
- RT-PCR analysis showed changes in cyclins and CKIs at the mRNA level.
- Transfection of EWS-Fli1 cDNA caused transformation of NIH3T3 cells.
Takeaway
The EWS-Fli1 gene can change how certain proteins that control the cell cycle are made, which might help tumors grow.
Methodology
The study involved treating cells with EWS-Fli1 antisense oligonucleotides and analyzing gene expression changes.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website