Dynamic variations in the peripheral blood lymphocyte subgroups of patients with 2009 pandemic H1N1 swine-origin influenza A virus infection
2011

Changes in Blood Cells of H1N1 Patients

Sample size: 76 publication Evidence: moderate

Author Information

Author(s): Guo Xichao, Chen Yu, Li Xuefen, Kong Haishen, Yang Shigui, Ye Bo, Cui Dawei, Wu Wei, Li Lanjuan

Primary Institution: First Affiliated Hospital, School of Medicine, Zhejiang University

Hypothesis

The study aims to investigate the dynamic fluctuations of peripheral blood lymphocyte subgroups in patients infected with H1N1 swine-origin influenza A virus.

Conclusion

Patients showed different lymphocyte subgroup distributions between mild and severe cases, which might affect the incidence and development of 2009 H1N1.

Supporting Evidence

  • The frequency of NK cells decreased in all cases.
  • CD19+ B cells were increased in severe cases compared to controls.
  • In moderate cases, CD4+ and CD4+CD25+ T cells were higher than in severe cases at 1-2 days from onset.
  • Serum CRP levels were significantly higher in both severe and moderate groups compared to controls.

Takeaway

This study looked at how different types of blood cells change in people who get sick from H1N1, showing that those who are more seriously ill have different blood cell patterns than those who are less sick.

Methodology

The study measured T cells, B cells, NK cells, and regulatory T cells in 76 H1N1 patients and 20 healthy controls using flow cytometry and analyzed serum cytokines and CRP levels.

Limitations

The study did not include immunomodulators in treatment and had a limited follow-up period.

Participant Demographics

The study included 76 patients (36 severe and 40 moderate cases) and 20 healthy volunteers, predominantly adults.

Statistical Information

P-Value

0.006

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/1743-422X-8-215

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