Adoption of an “Open” Envelope Conformation Facilitating CD4 Binding and Structural Remodeling Precedes Coreceptor Switch in R5 SHIV-Infected Macaques
2011

How HIV Changes to Use Different Cell Receptors

Sample size: 2 publication 10 minutes Evidence: moderate

Author Information

Author(s): Zhuang Ke, Finzi Andres, Tasca Silvana, Shakirzyanova Madina, Knight Heather, Westmoreland Susan, Sodroski Joseph, Cheng-Mayer Cecilia

Primary Institution: Aaron Diamond AIDS Research Center, New York, New York, United States of America

Hypothesis

An early step in the evolution of tropism switch is the adoption of a less constrained and more 'open' envelope conformation for better CD4 usage.

Conclusion

R5 viruses evolved to become more sensitive to CD4 binding, which correlated with better infection of CD4low cells and changes in neutralization sensitivity.

Supporting Evidence

  • R5 viruses became increasingly sensitive to CD4 binding prior to coreceptor switch.
  • The study observed significant changes in neutralization sensitivity close to the time of X4 emergence.
  • Increased sCD4 sensitivity correlated with better infection of CD4low cells.

Takeaway

HIV can change how it enters cells, and this study shows that it first becomes better at using a specific receptor before switching to another one.

Methodology

The study used R5 SHIV-infected macaques to assess changes in virus sensitivity to CD4 and coreceptor usage over time.

Participant Demographics

Two rapid progressor macaques infected with R5 SHIV.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0021350

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