Measuring Protein Concentration with NanoDrop Spectrophotometer
Author Information
Author(s): Philippe Desjardins, Joel B. Hansen, Michael Allen
Primary Institution: Thermo Scientific NanoDrop Products
Conclusion
The NanoDrop 2000c Spectrophotometer allows for accurate protein concentration measurements using very small sample volumes without the need for cuvettes.
Supporting Evidence
- The NanoDrop 2000c can measure protein concentrations as high as 400 mg/mL.
- The method eliminates the need for dilutions, increasing measurement speed and dynamic range.
- Microvolume measurements are crucial for modern molecular techniques that require small sample sizes.
Takeaway
This study shows how scientists can measure tiny amounts of proteins quickly and easily using a special machine that doesn't need traditional containers.
Methodology
The study describes protocols for measuring protein concentration using A280 absorbance and colorimetric assays with the NanoDrop 2000c.
Limitations
The accuracy of measurements can be affected by sample homogeneity and the need for proper cleaning and conditioning of the measurement surfaces.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website