Torque Teno Sus Virus in Cell Cultures and Trypsin
Author Information
Author(s): Teixeira Thais Fumaco, Dezen Diogenes, Cibulski Samuel Paulo, Varela Ana Paula Muterle, Holz Carine Lidiane, Franco Ana Cláudia, Roehe Paulo Michel
Primary Institution: Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), Brazil
Hypothesis
Is Torque Teno Sus Virus (TTSuV) present in cell cultures and trypsin used for cell disaggregation?
Conclusion
The study found that TTSuV genomes were present in several cell lineages and one batch of trypsin, indicating contamination risks.
Supporting Evidence
- Fifteen out of twenty-five cell lineages tested contained amplifiable TTSuV genomes.
- One batch of trypsin was found to contain TTSuV genomes.
- The presence of TTSuV in an ampoule frozen in 1985 suggests long-term contamination.
Takeaway
The researchers looked for a virus in cell cultures and found it in some cells and in trypsin, which could make other experiments unsafe.
Methodology
DNA from 25 cell lineages, sera, and trypsin was tested for TTSuV using duplex PCR.
Potential Biases
Potential bias in sample selection and the possibility of residual contamination affecting results.
Limitations
The study did not test all possible sources of contamination and relied on specific PCR methods that may miss other variants.
Digital Object Identifier (DOI)
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