Study of the PTEN Gene in Breast Cancer
Author Information
Author(s): Feilotter H E, Coulon V, McVeigh J L, Boag A H, Dorion-Bonnet F, Duboué B, Latham W C W, Eng C, Mulligan L M, Longy M
Primary Institution: Department of Pathology, Queen's University, Kingston, Ontario, Canada
Hypothesis
Is there a loss of heterozygosity in the PTEN gene in sporadic breast carcinoma?
Conclusion
Loss of heterozygosity of the PTEN locus is common in sporadic breast carcinoma, but mutations in the PTEN gene are not present.
Supporting Evidence
- 27 out of 70 breast tumor samples showed loss of heterozygosity in the PTEN region.
- The most commonly deleted region was flanked by markers D10S215 and D10S541.
- No mutations of the PTEN gene were detected in tumors with loss of heterozygosity.
Takeaway
The study looked at breast cancer samples and found that while a part of the PTEN gene was often missing, the gene itself wasn't broken.
Methodology
The study used denaturing gradient gel electrophoresis and single-strand conformation polymorphism analyses to investigate PTEN mutations.
Digital Object Identifier (DOI)
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