The antibacterial activity of a prophage-encoded fitness factor is neutralized by two cognate immunity proteins
2024

Bxa: A Bacterial Toxin with Antibacterial Properties

publication Evidence: high

Author Information

Author(s): Alexei Andrea G., Bullen Nathan P., Garrett Stephen R., Sychantha David, Whitney John C.

Primary Institution: McMaster University

Hypothesis

How do Bxa-producing bacteria resist intoxication prior to Bxa’s release from cells?

Conclusion

Bxa is a promiscuous ADP-ribosyltransferase that can kill bacteria, but its activity is neutralized by two immunity proteins.

Supporting Evidence

  • Bxa was shown to ADP-ribosylate non-muscle myosin II within host epithelial cells.
  • Bxa's antibacterial activity can be neutralized by the immunity protein BAH.
  • Bxa is encoded alongside immunity proteins that protect against its own toxicity.

Takeaway

Bxa is a toxin made by some bacteria that can harm other bacteria, but it has helpers that protect it from hurting itself.

Methodology

The study involved expressing Bxa in E. coli and assessing its antibacterial activity and the role of immunity proteins.

Limitations

The study did not explore the full range of potential targets for Bxa's activity in other bacterial species.

Digital Object Identifier (DOI)

10.1016/j.jbc.2024.108007

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