Alteration in Superoxide Dismutase 1 Causes Oxidative Stress and p38 MAPK Activation Following RVFV Infection
2011

Impact of Rift Valley Fever Virus on Antioxidant Protein SOD1 and Cellular Stress Responses

publication 10 minutes Evidence: moderate

Author Information

Author(s): Narayanan Aarthi, Popova Taissia, Turell Michael, Kidd Jessica, Chertow Jessica, Popov Serguei G., Bailey Charles, Kashanchi Fatah, Kehn-Hall Kylene

Primary Institution: National Center for Biodefense and Infectious Diseases, George Mason University

Hypothesis

Does Rift Valley fever virus infection alter the levels of superoxide dismutase 1 and activate stress response pathways in human cells?

Conclusion

Rift Valley fever virus infection leads to decreased levels of the antioxidant protein SOD1 and activates the p38 MAPK stress response in human cells.

Supporting Evidence

  • SOD1 levels decreased significantly in infected cells compared to uninfected controls.
  • Increased oxidative stress was observed in cells infected with RVFV.
  • Activation of the p38 MAPK pathway was noted in response to RVFV infection.

Takeaway

When a virus infects human cells, it can make the cells stressed and lower their defenses, which can lead to more damage.

Methodology

Human small airway epithelial cells were infected with Rift Valley fever virus strains and analyzed for changes in SOD1 levels and p38 MAPK activation using western blot and RT-PCR.

Limitations

The study primarily focuses on specific cell types and may not represent all human cells.

Statistical Information

P-Value

p<0.00005

Statistical Significance

p<0.01

Digital Object Identifier (DOI)

10.1371/journal.pone.0020354

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