Identification of DAF-12 Target Genes by ChIP-chip
2011

DAF-12 Regulates a Network of Genes for Developmental Decisions

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Author Information

Author(s): Hochbaum Daniel, Zhang Yue, Stuckenholz Carsten, Labhart Paul, Alexiadis Vassili, Martin René, Knölker Hans-Joachim, Fisher Alfred L.

Primary Institution: University of Pittsburgh

Hypothesis

DAF-12 plays a role in developmental robustness by regulating target genes involved in developmental decisions.

Conclusion

DAF-12 ensures developmental robustness by regulating a network of genes that respond to environmental and genetic variations.

Supporting Evidence

  • DAF-12 binds to 1,175 genomic regions, indicating a broad regulatory role.
  • Target genes include those involved in developmental progression and microRNA regulation.
  • DAF-12 mutants show enhanced sensitivity to environmental fluctuations.

Takeaway

DAF-12 is like a coach that helps worms make important decisions about their growth and development, especially when things get tough.

Methodology

Chromatin immunoprecipitation followed by hybridization to whole-genome tiling arrays was used to identify DAF-12 target genes.

Limitations

The study primarily focuses on DAF-12's role in C. elegans, which may not directly translate to other organisms.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pgen.1002179

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