How Cell Adherence Affects Fibronectin Assembly
Author Information
Author(s): Xu Jielin, Bae Eunnyung, Zhang Qinghong, Annis Douglas S., Erickson Harold P., Mosher Deane F.
Primary Institution: University of Wisconsin-Madison
Hypothesis
Interactions of adherent cells with regions of adsorbed fibronectin other than modules 7F3-10F3 are required for early display of the cell surface sites that initiate and direct fibronectin assembly.
Conclusion
1F3 acts together with C-terminal modules to induce display of fibronectin assembly sites on adherent cells.
Supporting Evidence
- Fibronectin-null cells assemble exogenous fibronectin when adherent to intact fibronectin but not to its cell-binding domain.
- Coatings of proteolytic fibronectin fragments were tested for effects on fibronectin assembly.
- 1F3 and C-terminal modules are necessary for optimal supportive activity in fibronectin assembly.
Takeaway
Cells need certain parts of fibronectin to stick together and form a structure. If they don't have these parts, they can't build the fibronectin properly.
Methodology
Fibronectin-null fibroblasts were tested for fibronectin assembly on various fibronectin fragments and recombinant proteins.
Limitations
The study primarily focuses on fibronectin-null cells, which may not represent all cell types.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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