Viral Mimicry of Cdc2/Cyclin-Dependent Kinase 1 Mediates Disruption of Nuclear Lamina during Human Cytomegalovirus Nuclear Egress
2009
How a Virus Mimics a Host Enzyme to Escape the Nucleus
publication
10 minutes
Evidence: high
Author Information
Author(s): Hamirally Sofia, Kamil Jeremy P., Ndassa-Colday Yasmine M., Lin Alison J., Jahng Wan Jin, Baek Moon-Chang, Noton Sarah, Silva Laurie A., Simpson-Holley Martha, Knipe David M., Golan David E., Marto Jarrod A., Coen Donald M.
Primary Institution: Harvard Medical School
Hypothesis
UL97 phosphorylates lamin A/C to mediate nuclear egress during HCMV infection.
Conclusion
The study shows that UL97 directly phosphorylates lamin A/C, facilitating nuclear egress of HCMV.
Supporting Evidence
- UL97 phosphorylates lamin A/C at Ser22, crucial for nuclear lamina disassembly.
- Inhibition of UL97 reduces lamin A/C phosphorylation by approximately 50%.
- Morphological changes in the nucleus during HCMV infection depend on UL97 activity.
Takeaway
The virus HCMV uses a special protein to poke holes in the cell's nuclear wall, helping it escape and spread.
Methodology
The study used mass spectrometry to analyze phosphorylation of lamin A/C in HCMV-infected cells.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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