Evaluating Group B Streptococcus Detection Methods
Author Information
Author(s): Nicolette Teese, Daneeta Henessey, Christopher Pearce, Nigel Kelly, Suzanne Garland
Primary Institution: The Royal Women's Hospital
Hypothesis
Does delayed inoculation into transport media affect the detection rate of Group B Streptococcus?
Conclusion
Delayed inoculation into selective enrichment broth after transport in Amies medium maintains acceptable GBS detection rates.
Supporting Evidence
- 92.2% of isolates were detected in all tubes and at all times.
- 3.9% of isolates were negative until 2 hours delayed inoculation.
- 1.9% of isolates gave inconsistent results due to low inoculum.
Takeaway
This study shows that it's okay to keep samples in a special transport medium for up to 24 hours before testing for a bacteria called Group B Streptococcus.
Methodology
Clinical isolates of GBS were tested using direct inoculation and delayed inoculation from transport medium at various time intervals.
Limitations
The study design may have confounded results due to varying transport times and inoculum dilution.
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