Ribosome binding of phasiRNA precursors accelerates the 24-nt phasiRNA burst in meiotic maize anthers
2024
Ribosome Binding Promotes phasiRNA Biogenesis
publication
Author Information
Author(s): Yingjia Han, Jiang S, Dong X, Dai X, Wand S, Zheng Y, Yan G, Li S, Wu L, Walbot V
Primary Institution: University of Wisconsin, Madison, WI, USA
Hypothesis
Does ribosome binding play a role in the production of mature phasiRNA molecules during maize anther development?
Conclusion
Ribosome binding is important for the biogenesis of mature 24-nt phasiRNAs in maize anthers.
Supporting Evidence
- Ribosome binding and phasiRNA precursor levels peaked at the same stage in anther development.
- Mutations that impaired ribosome recognition led to a significant reduction in mature phasiRNAs.
- The study suggests that ribosome binding contributes to phasiRNA processing.
Takeaway
Ribosomes help make a special type of RNA in plants that is important for their growth and development.
Methodology
The study used ribosome profiling, small RNA sequencing, and RNA sequencing to analyze phasiRNA precursors in developing maize anthers.
Digital Object Identifier (DOI)
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