Rapid Mutation of Zebrafish Genes Using Zinc Finger Nucleases
Author Information
Author(s): Jonathan E. Foley, Jing-Ruey J. Yeh, Morgan L. Maeder, Deepak Reyon, Jeffry D. Sander, Randall T. Peterson, J. Keith Joung
Primary Institution: Massachusetts General Hospital
Hypothesis
Can the OPEN method be used to efficiently generate zinc finger nucleases (ZFNs) for modifying zebrafish genes?
Conclusion
The study demonstrates that the OPEN method can efficiently generate ZFNs that induce targeted mutations in zebrafish genes.
Supporting Evidence
- OPEN ZFNs induced mutations in five different zebrafish genes with high efficiency.
- Mutations were transmitted through the germline in zebrafish.
- OPEN method allows for rapid generation of ZFNs for various gene targets.
Takeaway
Scientists found a quick way to change genes in zebrafish using special tools called zinc finger nucleases, which help them make precise edits in the DNA.
Methodology
The OPEN method was used to engineer zinc finger nucleases for five zebrafish genes, which were then tested for their ability to induce mutations in somatic cells and germline transmission.
Limitations
The study does not address potential off-target effects of the ZFNs.
Digital Object Identifier (DOI)
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