Early Expression of CD64 and CD163 on Monocytes in Cardiac Surgery
Author Information
Author(s): Martina Kolackova, Manuela Kudlova, Pavel Kunes, Vladimir Lonsky, Jiri Mandak, Ctirad Andrys, Karolina Jankovicova, Jan Krejsek
Primary Institution: Medical School in Hradec Králové, Charles University in Prague
Hypothesis
The study aims to assess the kinetics of CD64 and CD163 expression on peripheral blood cells of cardiac surgical patients and the effect of cardiac bypass on the inflammatory response.
Conclusion
The expression of CD64 on monocytes increases earlier than on granulocytes in cardiac surgical patients, with higher CD163 levels in 'on-pump' patients.
Supporting Evidence
- The density of CD64 on monocytes peaked on the 1st postoperative day.
- CD163 levels were significantly higher in 'on-pump' patients compared to 'off-pump' patients.
- Granulocyte CD64 expression returned to baseline by the 7th postoperative day.
Takeaway
After heart surgery, certain markers on blood cells change quickly, showing how the body reacts to surgery, especially in patients who had their heart stopped during the operation.
Methodology
Forty patients were randomly assigned to CABG surgery with either 'on-pump' or 'off-pump' techniques, and flow cytometry was used to measure receptor expression.
Potential Biases
Potential bias due to the small sample size and the exclusion of one patient who died post-surgery.
Limitations
The study did not account for all potential confounding factors, such as variations in patient treatment protocols.
Participant Demographics
31 males and 9 females, mean age 67.6 years.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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