Creating a Maize cDNA Library Using Baculovirus
Author Information
Author(s): Meller Harel Helene Y, Fontaine Veronique, Chen Hongying, Jones Ian M, Millner Paul A
Primary Institution: University of Leeds
Hypothesis
Can a baculovirus display methodology be effectively used to construct a cDNA library from maize seedlings?
Conclusion
The maize cDNA library constructed in this study relies on the novel technology of baculovirus display and is unique in currently published cDNA libraries.
Supporting Evidence
- The library contained a total of 2.5 × 105 independent clones.
- Expression of two known maize proteins, calreticulin and auxin binding protein (ABP1), was confirmed.
- The methodology allows for the identification and isolation of proteins from maize.
Takeaway
The researchers made a special library of maize genes using a virus, which helps in finding important proteins in maize.
Methodology
Total RNA from young maize seedlings was reverse transcribed to cDNA and cloned into a baculovirus transfer vector, followed by infection of insect cells.
Limitations
The library was not grown further to avoid degeneration, which may limit the diversity of the library.
Digital Object Identifier (DOI)
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