Ara-LAM and Its Role in Leishmania Infection
Author Information
Author(s): Parna Bhattacharya, Gaurav Gupta, Saikat Majumder, Anupam Adhikari, Sayantan Banerjee, Kuntal Halder, Suchandra Majumdar, Moumita Ghosh, Shubho Chaudhuri, Syamal Roy, Subrata Majumdar
Primary Institution: Division of Molecular Medicine, Bose Institute, Kolkata, India
Hypothesis
Can Arabinosylated Lipoarabinomannan (Ara-LAM) regulate the immune response in Leishmania donovani infected macrophages through MAPK signaling?
Conclusion
Ara-LAM skews the immune response from an anti-inflammatory to a pro-inflammatory state by regulating IL-10 and IL-12 production through MAPK signaling.
Supporting Evidence
- Ara-LAM activates p38 MAPK signaling while inhibiting ERK signaling in infected macrophages.
- IL-12 production is enhanced while IL-10 production is reduced in Ara-LAM treated macrophages.
- Histone modifications at the IL-10 and IL-12 promoter regions were observed following Ara-LAM treatment.
- STAT3 and SOCS3 were identified as key regulators in the immune response modulation by Ara-LAM.
Takeaway
This study shows that a substance called Ara-LAM helps the body's immune system fight off a parasite by changing how certain immune signals work.
Methodology
The study involved treating macrophages from BALB/C mice with Ara-LAM and analyzing the effects on MAPK signaling and cytokine production.
Participant Demographics
BALB/C mice were used for the experiments.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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