Improved Test for Detecting Sarocladium kiliense
Author Information
Author(s): Wong Yu-Ning, Hau Pak-Ting, Chau Eddie Chung-Ting, Ng Laam-Ching, Murillo Michaela, Fung Joshua, Po Wai-Wai, Yu Ray Chun-Wai, Kam Melody Kai-Ting, Tam Emily Wan-Ting, Tsang Chi-Ching, Chow Franklin Wang-Ngai, Perlin David S.
Primary Institution: Department of Health Technology and Informatics, The Hong Kong Polytechnic University
Hypothesis
Can the specificity of a colorimetric LAMP assay for Sarocladium kiliense be enhanced using additives?
Conclusion
The optimized LAMP assay successfully detects Sarocladium kiliense with high specificity and sensitivity, reducing false positives from closely related species.
Supporting Evidence
- The assay demonstrated high sensitivity and specificity for detecting S. kiliense.
- Combining pullulan and TMAC significantly improved assay performance.
- The optimized assay can detect as little as 10 pg of fungal DNA in 45 minutes.
Takeaway
Researchers created a new test to find a fungus called Sarocladium kiliense that can make people sick, and it works better than older tests.
Methodology
The study developed a colorimetric LAMP assay and tested the effects of two additives, pullulan and TMAC, to improve specificity.
Limitations
The assay may face challenges from nucleic acid amplification inhibitors present in environmental samples.
Digital Object Identifier (DOI)
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