How UV Stress Affects CTCF in Corneal Epithelial Cells
Author Information
Author(s): Wang Yumei, Lu Luo
Primary Institution: Department of Medicine, David Geffen School of Medicine, University of California Los Angeles
Hypothesis
The regulation of CTCF by extracellular stress signals is dependent upon activations of an oxidative stress-regulated protein Bcl-3.
Conclusion
The study reveals a novel mechanism for regulatory control of CTCF in UV stress-induced human corneal epithelial cells, which requires activation and formation of Bcl-3/p50 complex through a noncanonical NF-κB pathway.
Supporting Evidence
- Bcl-3 was activated by UV irradiation to interact with NF-κB p50.
- The Bcl-3/p50 complex suppressed CTCF promoter activity.
- Knocking down Bcl-3 mRNA abolished the inhibitory effect of UV irradiation on CTCF promoter activity.
Takeaway
When corneal cells are stressed by UV light, a protein called Bcl-3 helps turn down the activity of another protein called CTCF, which is important for cell function.
Methodology
The study involved experiments on human corneal epithelial cells to analyze the effects of UV stress on CTCF expression and the role of Bcl-3.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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