Using Boron-Doped Diamond Electrodes to Modify Lysozyme
Author Information
Author(s): Iniesta Jesús, Esclapez-Vicente María Deseada, Heptinstall John, Walton David J., Peterson Ian R., Mikhailov Victor A., Cooper Helen J.
Primary Institution: University of Alicante
Hypothesis
Can boron-doped diamond electrodes retain enzyme activity better than platinum electrodes during electro-oxidative nitration of lysozyme?
Conclusion
Boron-doped diamond electrodes retain lysozyme activity better than platinum electrodes during electro-oxidative nitration.
Supporting Evidence
- Boron-doped diamond electrodes showed better retention of lysozyme activity compared to platinum electrodes.
- The nitration sites on lysozyme were confirmed as Tyr23 and Tyr20.
- Electro-oxidative nitration was favored at higher pH levels.
Takeaway
This study shows that using special diamond electrodes helps keep an important protein, lysozyme, working better than using regular metal ones.
Methodology
The study involved electro-oxidative nitration of lysozyme using boron-doped diamond electrodes and comparing the results with platinum electrodes.
Limitations
The study does not address potential long-term stability of the boron-doped diamond electrodes in various conditions.
Digital Object Identifier (DOI)
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