In vitro model for studying bladder cancer invasion
Author Information
Author(s): Fujiyama C, Jones A, Fuggle S, Bicknell R, Cranston D, Harris A L
Primary Institution: Molecular Oncology Unit, ICRF, Institute of Molecular Medicine, John Radcliffe Hospital, Oxford
Hypothesis
Can an in vitro model of bladder cancer invasion using rat bladder be developed to test mechanisms and therapeutic inhibitors of invasion?
Conclusion
The study found that the urokinase system is important for bladder invasion and the model can be used to investigate other mechanisms and test drugs.
Supporting Evidence
- The model allows for tumor cell-extracellular matrix interactions.
- Better differentiated cell lines grew as stratified epithelium.
- Poorly differentiated cells invaded across a broad front.
- Invasion was not simply related to proliferation rate.
- Four compounds were tested for their ability to inhibit invasion.
Takeaway
Researchers created a lab model to study how bladder cancer cells invade and tested some drugs to see if they could stop this invasion.
Methodology
An in vitro model using de-epithelialized rat bladder was developed, and human bladder cancer cell lines were seeded to study invasion.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website