Regulation of the V-ATPase along the Endocytic Pathway
Author Information
Author(s): Lafourcade Céline, Sobo Komla, Kieffer-Jaquinod Sylvie, Garin Jérome, van der Goot F. Gisou
Primary Institution: Global Health Institute, Ecole Polytechnique Fédérale de Lausanne, Faculty of Life Sciences, Lausanne, Switzerland
Hypothesis
How is pH controlled along the endocytic pathway in mammalian cells?
Conclusion
The activity of the V-ATPase in the endocytic pathway is regulated by reversible association/dissociation and interaction with specific lipid environments.
Supporting Evidence
- The V-ATPase is crucial for acidifying endosomes, which is important for various cellular processes.
- Late endosomes have a higher V1/V0 ratio compared to early endosomes, correlating with increased acidity.
- Cholesterol-rich domains in membranes influence the activity of the V-ATPase.
Takeaway
This study shows that the acidity of late endosomes is higher because of more V-ATPase being attached to the membrane, and that the type of lipids around it also matters.
Methodology
The study involved proteomic analysis of detergent-resistant membranes from late endosomes and measurements of pH in late endosomes using fluorescence imaging.
Limitations
The study does not provide direct evidence that cholesterol extraction from the plasma membrane significantly reduces cholesterol in late endosomes.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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