Role of a Trypsin-like Protease in Pseudorabies Virus Invasion
Author Information
Author(s): Glorieux Sarah, Favoreel Herman W, Steukers Lennert, Vandekerckhove Annelies P, Nauwynck Hans J
Primary Institution: Ghent University
Hypothesis
The study investigates whether proteases are involved in the invasion of pseudorabies virus through the basement membrane.
Conclusion
A trypsin-like serine protease is significantly involved in the penetration of the pseudorabies virus through the basement membrane.
Supporting Evidence
- The serine protease-specific inhibitor AEBSF inhibited viral penetration by 88.1%.
- Using Soybean Type I-S trypsin inhibitor resulted in a 96.9% reduction in plaque depth.
- Proteases are classified into different types, and only serine protease inhibitors showed significant effects on viral penetration.
Takeaway
The study found that a specific type of enzyme helps the pseudorabies virus get through a barrier in the nose, which could help it spread in the body.
Methodology
The study used porcine nasal respiratory mucosal explants to analyze the effect of various protease inhibitors on viral penetration.
Limitations
The involvement of chymotrypsin-like and elastase-like serine proteases could not be fully examined due to the lack of observable plaques after treatment with specific inhibitors.
Participant Demographics
Porcine nasal respiratory mucosa explants from piglets.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website