Mapping H3K9 Methylation and Its Relationship with DNA Methylation in Arabidopsis
Author Information
Author(s): Yana V. Bernatavichute, Xiaoyu Zhang, Shawn Cokus, Matteo Pellegrini, Steven E. Jacobsen, Brian P. Dilkes
Primary Institution: University of California Los Angeles
Hypothesis
How does H3K9 methylation correlate with CHG DNA methylation in Arabidopsis thaliana?
Conclusion
H3K9m2 methylation is tightly correlated with CHG DNA methylation across the Arabidopsis genome.
Supporting Evidence
- H3K9m2 is highly enriched in pericentromeric regions of Arabidopsis chromosomes.
- H3K9m2 and CHG methylation are correlated throughout the genome.
- Distinct patterns of H3K9m2 exist in pericentromeric heterochromatin compared to euchromatic arms.
- Genes in pericentromeric regions show low expression levels when associated with H3K9m2.
Takeaway
This study looked at how a specific chemical change on proteins in plants relates to another type of chemical change on DNA, finding that they are closely linked.
Methodology
The study used chromatin immunoprecipitation coupled with high-resolution tiling arrays to map H3K9m2 methylation across the Arabidopsis genome.
Limitations
The study primarily focuses on Arabidopsis and may not be generalizable to other species.
Digital Object Identifier (DOI)
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