Aqueous Extract of Shi-Liu-Wei-Liu-Qi-Yin Induces G2/M Phase Arrest and Apoptosis in Human Bladder Carcinoma Cells via Fas and Mitochondrial Pathway
2011

Shi-Liu-Wei-Liu-Qi-Yin and Its Effects on Bladder Cancer Cells

Sample size: 4 publication 10 minutes Evidence: moderate

Author Information

Author(s): Ting-Tsz Ou, Wang Chau-Jong, Hung Guang-Uei, Wu Cheng-Hsun, Lee Huei-Jane

Primary Institution: Chung Shan Medical University

Hypothesis

The study aims to clarify the possible mechanisms of Shi-Liu-Wei-Liu-Qi-Yin (SLWLQY) in treating bladder cancer.

Conclusion

SLWLQY reduces tumor growth and induces cell-cycle arrest and apoptosis in human bladder carcinoma cells through the Fas and mitochondrial pathways.

Supporting Evidence

  • SLWLQY treatment resulted in G2/M phase arrest in TSGH-8301 cells.
  • Apoptotic cell death was confirmed by increased levels of caspases.
  • SLWLQY significantly reduced tumor size in a mouse model.
  • Fas and FasL protein expressions were up-regulated after SLWLQY treatment.

Takeaway

This study found that a traditional herbal medicine can help stop cancer cells from growing and make them die.

Methodology

The study used MTT assays for cytotoxicity, cell-cycle analysis, and western blot analysis on TSGH-8301 cells and a mouse model.

Participant Demographics

The study involved human bladder carcinoma TSGH-8301 cells and BALB/c nude mice.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1093/ecam/nep016

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