Simultaneous determination of eight major bioactive compounds in Dachengqi Tang (DT) by high-performance liquid chromatography
2008
New Method for Analyzing Key Ingredients in Dachengqi Tang
Sample size: 3
publication
Evidence: high
Author Information
Author(s): Tang Wenfu, Wan Meihua, Zhu Zhengyan, Chen Guanyuan, Huang Xi
Primary Institution: West China Hospital, Sichuan University
Hypothesis
A reliable analytical method for quality control of Dachengqi Tang (DT) is needed.
Conclusion
A reliable HPLC method for simultaneous determination of the eight markers in DT was developed.
Supporting Evidence
- The method showed a linear relationship between the concentrations of the markers and the peak area ratio.
- The limit of detection ranged from 0.21 to 0.43 ng/μl.
- The recovery was between 95.6% and 103.4%.
- The profiles of the markers did not vary significantly among batches.
Takeaway
Researchers created a new way to check the important ingredients in a traditional Chinese medicine called Dachengqi Tang, making sure it works well.
Methodology
A high-performance liquid chromatography (HPLC) method was developed to analyze eight markers in DT.
Limitations
The study only examined local herbs from Sichuan, China.
Statistical Information
P-Value
<0.001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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