A comparative evaluation of various invasion assays testing colon carcinoma cell lines
1999
Testing Invasion Assays for Colon Cancer Cells
publication
Author Information
Author(s): N J de Vermey, W N Dinjens, F T Bosman
Primary Institution: Erasmus University Medical Center
Hypothesis
The study evaluates the invasive capacity of various colon carcinoma cell lines using different assays.
Conclusion
The invasive capacity of neoplastic cells in vitro is highly dependent on the test system used.
Supporting Evidence
- None of the human colon carcinoma cells migrated through porous membranes coated with Matrigel.
- Only the murine line Colon-26 invaded clearly confluent fibroblasts and chicken heart tissue.
- Invasive capacity in vitro is strongly correlated with spindle cell shape and vimentin expression.
Takeaway
Different tests show how well colon cancer cells can invade, and the results can change based on the test used.
Methodology
Various colon carcinoma cell lines were tested in different invasion assays, including Matrigel and fibroblast layers.
Digital Object Identifier (DOI)
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