Impact of CaNa2EDTA fortification on growth, antioxidant activity and stress-related gene expression in tilapia (Oreochromis niloticus) at different stocking densities paradigms
2025

Effects of EDTA on Growth and Stress in Tilapia

Sample size: 4200 publication 10 minutes Evidence: moderate

Author Information

Author(s): Komal Wajeeha, Fatima Shafaq, Minahal Qandeel, Liaqat Razia, Abdul Latif Asma, Hussain Aya S.

Primary Institution: Lahore College for Women University, Lahore, Punjab, Pakistan

Hypothesis

How does CaNa2EDTA supplementation affect growth, immune response, and antioxidant activity in Nile tilapia at different stocking densities?

Conclusion

EDTA supplementation improved growth and antioxidant response in tilapia, with the best results seen at a dose of 5 g/kg in the high-density group.

Supporting Evidence

  • Fish in the low-density group showed better growth than those in medium and high-density groups.
  • Antioxidant enzyme levels were higher in the high-density group compared to the low-density group.
  • EDTA supplementation at 5 g/kg resulted in the best growth performance.
  • Significant changes in the chemical composition of the fish were observed across all treatments.

Takeaway

Adding a special ingredient called EDTA to fish food helps tilapia grow better and stay healthier, especially when they are kept close together.

Methodology

The study involved raising tilapia at three different stocking densities and feeding them diets with varying levels of EDTA supplementation for 60 days.

Limitations

The study did not explore the long-term effects of EDTA supplementation beyond the 60-day trial period.

Participant Demographics

Nile tilapia (Oreochromis niloticus) were used in the study.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0316629

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