Evaluating Petrifilm™ for Detecting Resistant E. coli
Author Information
Author(s): Wu Shuyu, Chouliara Eirini, Jensen Lars Bogø, Dalsgaard Anders
Primary Institution: Technical University of Denmark
Hypothesis
Can the 3M™ Petrifilm™ Select E. coli Count Plate effectively discriminate between antimicrobial-resistant and non-resistant E. coli?
Conclusion
The SEC plates can effectively screen and discriminate resistant E. coli for several antimicrobials but are not suitable for sulphamethoxazole.
Supporting Evidence
- SEC plates showed 74% agreement with Mueller-Hinton II media.
- High agreement levels for gentamicin, ampicillin, chloramphenicol, and cefotaxime.
- SEC plates can discriminate oxytetracycline-resistant E. coli with a breakpoint of 64 mg/L.
- SEC plates were not suitable for sulphamethoxazole-resistant E. coli.
Takeaway
This study tested a special plate to see if it could tell the difference between sick and healthy E. coli bacteria, and it worked well for most types but not for one specific kind.
Methodology
E. coli isolates were tested using agar dilution method to compare MICs across different media.
Limitations
Limited number of isolates for each antimicrobial and inoculum effect not evaluated.
Participant Demographics
Isolates were from pigs or cattle.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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