Expression of Carbonic Anhydrases IX and XII During Mouse Embryonic Development
Author Information
Author(s): Heini Kallio, Silvia Pastorekova, Jaromir Pastorek, Abdul Waheed, William S Sly, Susanna Mannisto, Markku Heikinheimo, Seppo Parkkila
Primary Institution: Institute of Medical Technology, University of Tampere and Tampere University Hospital
Hypothesis
Are carbonic anhydrases IX and XII expressed in mouse embryos during different stages of development?
Conclusion
Membrane-bound CA isozymes IX and XII are expressed in various tissues during mouse organogenesis.
Supporting Evidence
- CA IX and XII are present in several tissues of the developing mouse embryo during organogenesis.
- CA IX showed moderate signals in the brain, lung, pancreas, and liver.
- CA XII was most prominently stained in the choroid plexus of the brain.
Takeaway
This study found that two important enzymes, CA IX and CA XII, are present in different parts of developing mouse embryos, which might help keep the embryo's environment stable.
Methodology
Immunohistochemistry was used to detect the presence of CA IX and CA XII in mouse embryonic tissues.
Limitations
The study does not provide functional evidence that CA IX or XII is involved in cell migration during embryogenesis.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website