Dynamics of Core Planar Polarity Protein Turnover and Stable Assembly into Discrete Membrane Subdomains
2011
How Core Proteins Organize in Cell Junctions
publication
Evidence: high
Author Information
Author(s): Helen Strutt, Samantha J. Warrington, David Strutt
Primary Institution: MRC Centre for Developmental and Biomedical Genetics, University of Sheffield
Hypothesis
The core planar polarity proteins assemble into intercellular complexes at adherens junctions.
Conclusion
Core planar polarity proteins localize stably to discrete membrane puncta, which are essential for generating cellular asymmetry.
Supporting Evidence
- Core polarity proteins form asymmetric cell-cell contacts at discrete membrane puncta.
- Stable junctional localization of Flamingo and Frizzled occurs without cytoplasmic core proteins.
- Cytoplasmic core proteins promote concentration of Flamingo and Frizzled into puncta.
- Puncta are an important level of protein organization required for cellular asymmetry.
Takeaway
This study shows that certain proteins in cells stick together in special spots to help cells know which way to point.
Methodology
The study used antibody internalization assays and fluorescence recovery after photobleaching (FRAP) to analyze protein turnover.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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