Streamlined protein expression and purification using cleavable self-aggregating tags
2011
Streamlined Protein Expression and Purification Using Self-Aggregating Tags
publication
Evidence: moderate
Author Information
Author(s): Xing Lei, Wu Wei, Zhou Bihong, Lin Zhanglin
Primary Institution: Tsinghua University
Hypothesis
Can self-assembling amphipathic peptides improve protein expression and purification in E. coli?
Conclusion
The new purification method produces proteins with high quantity and purity while reducing costs and time.
Supporting Evidence
- The method produced proteins with over 90% purity.
- Yields ranged from 1.6 to 10.4 μg/mg wet cell pellet.
- The approach eliminates the need for costly affinity resins.
Takeaway
Scientists found a simpler way to make and clean proteins using special tags that help them stick together, making it easier to get the good stuff out.
Methodology
The study used self-aggregating peptides and intein-mediated cleavage for protein purification.
Limitations
The use of DTT for cleavage may limit the types of proteins that can be purified.
Digital Object Identifier (DOI)
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