Reference Ranges for the Clinical Laboratory Derived from a Rural Population in Kericho, Kenya
2008

Clinical Reference Ranges for a Rural Population in Kericho, Kenya

Sample size: 1293 publication 10 minutes Evidence: high

Author Information

Author(s): Kibaya Rukia S., Bautista Christian T., Sawe Frederick K., Shaffer Douglas N., Sateren Warren B., Scott Paul T., Michael Nelson L., Robb Merlin L., Birx Deborah L., de Souza Mark S.

Primary Institution: Walter Reed Project, U. S. Military HIV Research Program, Kericho, Kenya

Hypothesis

The study aims to develop region-specific clinical reference ranges for laboratory tests in a rural population in Kenya to support HIV vaccine trials.

Conclusion

The study establishes the first clinical reference ranges for a highland community in Kenya, highlighting significant differences compared to North American populations.

Supporting Evidence

  • The median CD4+ T cell count for the group was 810 cells/µl.
  • Kenyan subjects had lower median hemoglobin concentrations compared to North Americans.
  • There were significant gender differences for both red and white blood cell parameters.
  • Clinical chemistry reference ranges were comparable to those from the USA, with some exceptions.

Takeaway

This study found that the normal blood test values for people in Kericho, Kenya, are different from those in the US, which is important for treating and studying HIV.

Methodology

Lymphocyte immunophenotyping and hematology were performed on 1293 HIV seronegative participants, with clinical chemistry tests on up to 1541 individuals.

Potential Biases

Potential bias due to the exclusion of febrile, pregnant, or HIV seropositive individuals from the study.

Limitations

The study did not include a stool parasitologic survey for intestinal helminths, which may affect eosinophil counts.

Participant Demographics

Participants were aged 18 to 55 years, predominantly from the Kalenjin, Kisii, Luo, and Luhya tribes.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0003327

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