Transgenerational Epigenetic Programming of the Brain Transcriptome and Anxiety Behavior
2008

Transgenerational Effects of Vinclozolin on Brain and Behavior

Sample size: 12 publication 10 minutes Evidence: moderate

Author Information

Author(s): Michael K. Skinner, Matthew D. Anway, Marina I. Savenkova, Andrea C. Gore, David Crews

Primary Institution: Washington State University

Hypothesis

Does embryonic exposure to vinclozolin lead to transgenerational changes in brain transcriptomes and anxiety behavior?

Conclusion

Embryonic exposure to vinclozolin causes distinct transgenerational changes in brain development and behavior, with males showing decreased anxiety-like behavior and females showing increased anxiety-like behavior.

Supporting Evidence

  • Vinclozolin exposure altered the expression of 92 genes in the male hippocampus and 1,301 genes in the female hippocampus.
  • Behavioral assays showed that male rats had decreased anxiety-like behavior while females had increased anxiety-like behavior.
  • Transgenerational effects were observed three generations after the initial exposure to vinclozolin.

Takeaway

If a mother rat is exposed to a chemical while pregnant, it can change how her babies' brains work and how they feel, even when they grow up.

Methodology

Gestating rats were injected with vinclozolin during specific embryonic days, and the F3 generation was analyzed for brain transcriptome changes and behavior.

Potential Biases

Potential bias in behavioral assessments due to environmental conditions during testing.

Limitations

The study primarily focuses on one chemical and its effects; other environmental factors were not considered.

Participant Demographics

Sprague-Dawley rats, both male and female, across multiple generations.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0003745

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