Mapping Mitotic Crossovers in Yeast
Author Information
Author(s): Lee Phoebe S., Greenwell Patricia W., Dominska Margaret, Gawel Malgorzata, Hamilton Monica, Petes Thomas D.
Primary Institution: Duke University Medical Center
Hypothesis
What are the properties and mechanisms of mitotic recombination in yeast?
Conclusion
Most spontaneous reciprocal crossovers in yeast are associated with long gene conversion tracts, with about 40% indicating a DNA double-strand break initiated in G1.
Supporting Evidence
- Most crossovers are associated with adjacent conversion tracts.
- Conversion tracts are often very long, averaging about 12 kb.
- About 40% of conversion events suggest repair of a chromosome broken in G1.
Takeaway
Yeast can swap pieces of their DNA during cell division, and this study shows that these swaps can be much longer than previously thought.
Methodology
The study used a genetic system to select for daughter cells containing the products of mitotic crossovers and mapped crossovers and gene conversion events in a specific chromosomal region.
Potential Biases
Potential bias in the selection of strains and the specific genetic markers used.
Limitations
The study primarily focuses on a specific chromosomal region and may not represent all mitotic recombination events in yeast.
Participant Demographics
The study involved yeast strains derived from two closely related haploids.
Statistical Information
P-Value
p<0.001
Confidence Interval
95% confidence limits
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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